I have a question about the interaction between a kinase dead version of a kinase and its substrates. My colleagues always told me that apparently the kinase dead version binds more tightly to a substrate than what would do the wild type, as if the kinase dead stays attached to the substrate trying to phosphorylate it (but it cannot..). Based on this idea if one immunoprecipitates the kinase dead version of a kinase, he should find more "real" substratesâ€¦Can you tell me whether this story is true? Or, better, do you know any scientific evidence about this?
I ask you this cause my colleagues do not know anyâ€¦and they say is more an unwritten rule/speculation rather than a real scientific proof.
Hope you can help!
If it doesn't fit into any other category post it here.
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