Paper comparing RP gradient lenghts

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TokeH
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Paper comparing RP gradient lenghts

Postby TokeH » Mon Jun 23, 2014 9:52 am

I remember seeing a paper showing a plot with peptide IDs as a function of RP gradient length, but cannot find it again. Anyone that can help?

Thanks

m/z
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Postby m/z » Tue Jun 24, 2014 10:25 am

I once saw a detailed presentation made by LCMS-experts from Thermo that compared gradient lengths, temperature, column lengths, and 2D vs 1D LCMS, etc with respect to number of protein and peptide IDs. I am pretty sure they will share it if you ask...

Zhang
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Postby Zhang » Fri Jun 27, 2014 8:35 pm

We estimated the relation between peptide/protein ID with LC column and gradient.
http://www.ncbi.nlm.nih.gov/pubmed/23878402

My feeling is that it should be a sample-related and instrument-dependent non-linear function.

TokeH
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Postby TokeH » Thu Jul 03, 2014 7:25 am

Hi Guys
Thanks for the replies.
@Zhang: I found a reference in your paper that also have data for >5 h gradient (23 min to 458 min gradients):
Cristobal, A., Hennrich, M. L., Giansanti, P., Goerdayal, S. S., Heck, A. J. R., & Mohammed, S. (2012). In-house construction of a UHPLC system enabling the identification of over 4000 protein groups in a single analysis. The Analyst, 137(15), 3541.


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