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GuHCl or Urea

Posted: Thu May 17, 2012 11:36 am
by Peace
Hi All,

I like to ask a question.

When we are purifying the proteins under denatured condition, we can use Urea or GuHCl (Guanidine hydrochloride). These both are strong denaturants .
What is the difference between them?

thanks

Posted: Sun May 20, 2012 11:54 pm
by Toxic
GuHCl is not compatible with IEF whereas Urea is. Otherwise, I don't see much difference in their ability as chaotropes.

Posted: Fri Dec 07, 2012 9:55 pm
by hughes
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Posted: Mon Dec 10, 2012 4:31 am
by Kelstrup
There was a comparison published recently. HERE