Wanted: Most stable molecule for HCD mass spectrometry

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Honeybee
Carbon Member
Carbon Member
Posts: 3
Joined: Thu Aug 09, 2012 11:04 am

Wanted: Most stable molecule for HCD mass spectrometry

Postby Honeybee » Sun Aug 26, 2012 10:40 am

Dear all,

I am looking for very stable molecules that do not get fragmented with Higher-energy collisional dissociation (HCD, at least at usual NCEs) and with a molecular weight larger than 300 Da. Furthermore they should be positively charged.

I know, polycyclic aromatic hydrocarbons might be interesting candidates...

Any suggestion is appreciated !

Best,

W.

Craig
E. Coli Lysate Member
E. Coli Lysate Member
Posts: 220
Joined: Sun Jun 26, 2011 6:49 pm

Postby Craig » Mon Aug 27, 2012 11:40 am

Have you tried Ultramark? It is common in tune/calibration mixtures and pretty stable as far as I know, though I've never tried HCD on it.

Craig
E. Coli Lysate Member
E. Coli Lysate Member
Posts: 220
Joined: Sun Jun 26, 2011 6:49 pm

Postby Craig » Mon Aug 27, 2012 3:18 pm

Maybe Ultramark isn't such a good idea...I found out that it is used for calibrating HCD collision energy!


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