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Proteomic methods detects proteins that interact with cholesterol

Posted: Mon Feb 11, 2013 9:21 am
by Doug
A new paper in Nature methods from Benjamin Cravatt's lab describes a method to detect metabolite-protein interactions. They basically use the metabolite (in this case sterols) as a a bait to pull down interacting proteins which are detected by mass spectrometry.

Proteome-wide mapping of cholesterol-interacting proteins in mammalian cells
Jonathan J Hulce, Armand B Cognetta, Micah J Niphakis, Sarah E Tully & Benjamin F Cravatt