As i wish to know how the Spectrum pattern and How the Phosphorylation Site can be identified from HCD Data,As in ETD or CID the Neutral Loss Peak intensity will be Highest Peak but in the case of HCD spectrum its Difficult to predict the Site Localization of Phosphate in peptide Sequence.
Is there any tool to work or basic concept to know about basic of Phosphopeptide Spectral pattern in HCD.
Thanks in advance
Integrative methods to study complex biological phenomena
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