Help with eletrophoresis

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Duque
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Joined: Thu Mar 19, 2015 6:01 pm

Help with eletrophoresis

Postby Duque » Thu Mar 19, 2015 6:34 pm

Hello!!

My name is July. I´m working in Inmunobloting of a chimera protein for diagnosis.
I have had problems with my gels because the proteins run as “zig-zagâ€￾ , these don’t run equally .
I have changed the buffers, the acrylamide but this don´t function.
The electrophoresis has the next conditions:
SDS-PAGE 12%
100 volt during 1 hour

Can you help me? Please
Thanks!!!

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zougman
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Joined: Mon Mar 10, 2014 11:27 pm

Postby zougman » Tue Mar 24, 2015 3:05 pm

Hi July,

If you are sure that your buffers are fine, the power supply functions OK, you do use the proper stacking gel on the top of the resolving gel
then the most plausible explanation for the Zig Zag pattern is high salt concentration in your sample/s.

Sincerely,

AZ

Duque
Proton Member
Proton Member
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Joined: Thu Mar 19, 2015 6:01 pm

Postby Duque » Wed Apr 01, 2015 8:43 am

Hi Az,

I have seen little bubbles during the electrophoresis.
I ran the electrophoresis during 6 hours, then I did the transference and it was fine.
I believe the electrode assembly module is bad.
Thanks for all.

Christopher
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Joined: Thu Dec 27, 2012 12:26 pm

Postby Christopher » Thu Apr 02, 2015 7:26 am

I agree with Zougman above. Sounds like it is a problem with the composition of your sample rather than a bad module.


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