How many p eller micro gram are presented in a single cell?

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Kambiz
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How many p eller micro gram are presented in a single cell?

Postby Kambiz » Sat Jun 21, 2014 7:59 pm

Hi everybody

I was wondering how many pico eller micro gram protein are expressed in a single cell? Please supply me with a paper or two.

Motashakeram
Kambiz
Kambiz Gilany, Ph.D.
Assistant Professor
Avicenna Research Institute (ARI)

Evin, Tehran, Iran
P.O. Box: 19615-1177

Tel: +98-21-22432020 Ext. 427
Fax: +98 21 22432021

Website: http://www.avicenna.ac.ir/index.php/en

Kambiz
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Joined: Tue Nov 29, 2011 12:34 am

Postby Kambiz » Sun Jun 22, 2014 8:07 pm

I have found a book by Fred Sherman "Guide to yeast genetics and molecular biology" (Methods in Enzmology vol 194) it is writin
that 6 pg/cell (haploids) and 8 pg/cell (dipolids). I think it is to low.

tsbatth
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Postby tsbatth » Tue Jun 24, 2014 1:21 pm

Depends on cell type and organism, would be different for each I would guess.

chengh1
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Postby chengh1 » Wed Jun 25, 2014 1:26 am

S. cerevisiae, S. pombe and HeLa cells contain approximately 3 pg/cell, 9 pg/cell and 200 pg/cell of protein, respectively. nature method .2834

Kambiz
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Posts: 43
Joined: Tue Nov 29, 2011 12:34 am

Postby Kambiz » Sat Jun 28, 2014 10:19 pm

Dear chengh1 Will you be nice and supply the reference. Nature method 2834? Is it reference?
Kambiz Gilany, Ph.D.
Assistant Professor
Avicenna Research Institute (ARI)

Evin, Tehran, Iran
P.O. Box: 19615-1177

Tel: +98-21-22432020 Ext. 427
Fax: +98 21 22432021

Website: http://www.avicenna.ac.ir/index.php/en

chengh1
Glycine Member
Glycine Member
Posts: 5
Joined: Tue Jun 24, 2014 11:12 pm

Postby chengh1 » Wed Jul 02, 2014 12:59 am

Hi Kambiz, yes, it is a reference. The link is ' http://www.nature.com/nmeth/journal/v11/n3/abs/nmeth.2834.html ' . Cheers, Cheng


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