I am a new comer to this group, and I hope I can get some discussions related to an observation we recently had on a AB 4800 MALDI TOF/TOF for peptide analysis, we are analyzing a plant peptide with sequence YCRHPSS (in which C is carbamidomethyled), I used 2kv MSMS with CID gas on, and oberved a strong (precursor-91)+ ion in the MSMS spectrum. It's hard to explain as Y usually loses 107 (arometic ring) during fragmenation, does anyone have ever observed similar phenomenon? any suggestions/comments will help. Thank you in advance.
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