ESI signal enhancer for peptide detection

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cavaliersun2003
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ESI signal enhancer for peptide detection

Postby cavaliersun2003 » Wed Nov 05, 2014 8:45 am

I just came across two very interesting papers talked about the signal improvement in ESI-MS for peptide analysis by DMSO!
http://link.springer.com/article/10.1007%2Fs13361-012-0404-0
http://www.nature.com/nmeth/journal/v10/n10/full/nmeth.2610.html
Does any one have tried this out in your intruments?
Or any other choice of ESI signal enhancer out there?

Craig
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Postby Craig » Wed Nov 05, 2014 8:57 am

This was discussed on this forum pretty extensively here.

PhilG
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Postby PhilG » Wed Nov 05, 2014 11:32 am

I recently met with a MS applications specialist who highly discouraged me from using DMSO in the HPLC solvents. The specialist indicated the DMSO could damage HPLC seals and fittings and thus severely diminish the HPLC's performance. Additionally, it sounds like the DMSO could coat the ion path of the mass spectrometer and reduce its sensitivity. Hearing this I am nervous to try it. I'd love to hear if anyone has any experience (good or bad) using DMSO in the HPLC solvents.

cavaliersun2003
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Postby cavaliersun2003 » Wed Nov 05, 2014 5:38 pm

PhilG wrote:I recently met with a MS applications specialist who highly discouraged me from using DMSO in the HPLC solvents. The specialist indicated the DMSO could damage HPLC seals and fittings and thus severely diminish the HPLC's performance. Additionally, it sounds like the DMSO could coat the ion path of the mass spectrometer and reduce its sensitivity. Hearing this I am nervous to try it. I'd love to hear if anyone has any experience (good or bad) using DMSO in the HPLC solvents.


thank you all.
Concerning the the possible detrimental effect of DMSO on HPLC, can we just add the DMSO post-column?
They mentioned to use 275C in ion-transfer tube temperature so the DMSO can be effectively desolved. I don't know if this make sense.
I will try it out at some point with QE and ELITE, anyone have try DMSO on these two Thermo instruments other than the original author of the paper?

tsbatth
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Postby tsbatth » Thu Nov 06, 2014 1:55 am

cavaliersun2003 wrote:thank you all.
Concerning the the possible detrimental effect of DMSO on HPLC, can we just add the DMSO post-column?
They mentioned to use 275C in ion-transfer tube temperature so the DMSO can be effectively desolved. I don't know if this make sense.
I will try it out at some point with QE and ELITE, anyone have try DMSO on these two Thermo instruments other than the original author of the paper?


The DMSO trick doesn't work with QE. By the time the paper had come out and the last ASMS, it seemed like most people had stopped using it. I also heard other stories of it ruining ion mobility agilent qtof systems. The conclusions derived on the long term impact on instrument performance from using DMSO seemed a bit insufficient given the small time frame (as people found out later).

cavaliersun2003
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Postby cavaliersun2003 » Thu Nov 06, 2014 4:50 pm

glad to hear so many dissucions about this DMSO trick before I put my instrument in an harm's way. I guess I will pass it. But just out of curiosity, why it won't work on QE?

tsbatth
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Postby tsbatth » Fri Nov 07, 2014 5:26 am

cavaliersun2003 wrote:glad to hear so many dissucions about this DMSO trick before I put my instrument in an harm's way. I guess I will pass it. But just out of curiosity, why it won't work on QE?


I'm unsure, but from what I recall they weren't sure either. They speculated it might have to do with the different design of the flatapole or S-lens or something like that.


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