I used integrated tip column before and just switched to separate ones due to special column. Has anyone met this unstable spray issue? I noticed very small bubbles coming out from the column and then get into the tip. I used long column and the pressure is high (600 bar) and this issue is understandable to me. Is there anyone know how to solve it? I tried to use a metal needle and it is better. But the chromatogram peak shape is still not as good as the integrated ones. Any suggestions? Thank you.
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- E. Coli Lysate Member
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