peak widths of phospho-peptides

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neil777
Angiotensin Member
Angiotensin Member
Posts: 27
Joined: Fri Jan 20, 2012 9:53 am

peak widths of phospho-peptides

Postby neil777 » Wed Jun 27, 2012 3:46 am

:) does anyone experience wider peak widths using C18 separation of phospho-peptides than for non phospho-peptides. If peak widths are broader for phosphos is there a way to improve it
best wishes
neil 777

JDRCHEM
Phosphoserine Member
Phosphoserine Member
Posts: 24
Joined: Wed Jun 29, 2011 5:35 am

Postby JDRCHEM » Wed Jun 27, 2012 6:34 am

Check out this thread. There are some very helpful suggestions for troubleshooting phosphopeptide separations.


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