Biomarker Validation : Triple Quad vs. Exactive vs. QTOF, etc.

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Biomarker Validation : Triple Quad vs. Exactive vs. QTOF, etc.

Postby mark.wingerd » Thu Jul 19, 2012 11:52 am

I have been asked to assess options for a new mass spec and LC-MS Systems.

As described to me, this system is primarily to be used for Proteomic Biomarker Verification / Validation.
Its main purpose is to be an "Orthogonal" approach to an Orbitrap XL in the Biomarker discover process.

It seems that classically Triple Quads have been used in this area of Biomarker Validation.
The current preference seems to be the SCIEX QTRAP 4500 System.

I have seen a little bit on the use of Orbitrap Exactive in this role.

I would like to get a community feedback on this.

- Is a Triple Quad seen as essential in this ?
- Could this be done with an Exactive ?
- Does the Exactive allow one to do other things that a Triple Quad cannot ?

This system is to augment our Orbitrap XL system.



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Postby RoyM » Fri Jul 20, 2012 12:19 pm

There are a lot of people looking at TOF's for this too. You've got to be pretty certain of what you're monitoring to use a TQ method, and then there are practical limits and the problems of interference. All of the techniques have tradeoffs; you need to do a thorough job setting up your experimental space to make the best decision.


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