HPLC question
-
- Proton Member
- Posts: 2
- Joined: Thu Dec 20, 2012 6:43 am
HPLC question
A colleague of mine has just suggested to me the possiblity that analytes (peptides or otherwise) can bind to one another rather than to the column when loaded i.e. peptide binds to another peptide which is bound to the column. Is this possible? I'd have guessed that the entropic cost of a peptide complexing with another peptide rather than associating with the column would be too great, but I'm really not sure.
-
- Proton Member
- Posts: 2
- Joined: Wed May 02, 2012 8:34 pm
in theory, it's possible
I would say it's possible in theory.
I did not testify that yet. However, based on some MRM experiment on standard peptides and complex peptide mixtures, I ever doubted that.
I did not testify that yet. However, based on some MRM experiment on standard peptides and complex peptide mixtures, I ever doubted that.
Randall wrote:A colleague of mine has just suggested to me the possiblity that analytes (peptides or otherwise) can bind to one another rather than to the column when loaded i.e. peptide binds to another peptide which is bound to the column. Is this possible? I'd have guessed that the entropic cost of a peptide complexing with another peptide rather than associating with the column would be too great, but I'm really not sure.
Who is online
Users browsing this forum: No registered users and 1 guest