Choice of standard QC proteins for spiking label-free quantitation experiments

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botte
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Joined: Mon Apr 29, 2013 11:47 pm

Choice of standard QC proteins for spiking label-free quantitation experiments

Postby botte » Wed May 14, 2014 12:23 am

Dear all,
I am looking for standard proteins that are readily commmercially available, not too expensive, are readily digested with trypsin, produce some suitable peptides for LC/MS/MS, and can be used as QC spikes for label-free quantitation to see if the analytical system is able to pick up defined concentration differences. Originally I was thinking of coming up with two sets:
Set A which can be used for spiking human/mammalian samples, so peptides should be largely unique in this "background":
Yeast Enolase, Yeast ADH, ... (?)
Set B which can be used for spiking bacterial samples, so unique against this "background":
BSA, Horse Cytochome C, ...(?)
Has anyone done this exercise before and can make some recommendations here?
Many thanks in advance!

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