Proline specific enzyme

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dimsam
Proton Member
Proton Member
Posts: 1
Joined: Mon Oct 06, 2014 7:35 am

Proline specific enzyme

Postby dimsam » Mon Oct 06, 2014 7:43 am

Hi everyone,

I am looking for enzymes that specifically cleave proteins either before or after Proline. Since the protein I am studing is more than 200 AAs long I cannot use, for example, proline endopeptidase. Are there any proline specific enzymes that can be used fordigestion larger molecules?

Thank you in advance.

Dim

cavaliersun2003
Ubiquitin Member
Ubiquitin Member
Posts: 50
Joined: Mon Jun 03, 2013 12:33 am

Postby cavaliersun2003 » Fri Oct 17, 2014 2:01 am

It really depends on what you want to do....
As I know the Prolyl endopeptidase can digest pepitde smaller than 10Kda. Can you use the other proteases, such as LysC to generate smaller sections of peptide fragments (which will skip KP) and then use prolyl endopeptidase to further digest it?


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