Nuclear phosphorpteomics

Share protocols and ask for sample preparation advice.
ncmm
Proton Member
Proton Member
Posts: 1
Joined: Sun Jan 17, 2016 9:33 am

Nuclear phosphorpteomics

Postby ncmm » Sun Jan 17, 2016 9:39 am

Hi All,
I want to perform nuclear phosphproteomics cancer cells (cell culture). Could some one suggest me a protocol for nuclear proteins extraction which can be further suitable for in-solution protein digestion?
thanks
ss

Biomarker
Albumin Member
Posts: 88
Joined: Thu Aug 09, 2012 3:28 am

Re: Nuclear phosphorpteomics

Postby Biomarker » Mon Jun 13, 2016 3:06 am

Hey,

You can use https://tools.thermofisher.com/content/sfs/manuals/MAN0011398_NEPER_Nuc_Cytoplasmic_Extract_Reag_UG.pdf for nuclear protein extraction. I am not sure about the recepie of buffers but may be once lysis is done, you can do buffer exchange of lysate with 50 mM ammonium bicarbonate followed by in solution digestion. Hope it helps.


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