Share protocols and ask for sample preparation advice.
2 posts • Page 1 of 1
- Ubiquitin Member
- Posts: 52
- Joined: Thu Dec 15, 2011 7:58 am
I have come across this modification (Gln->pyro-Glu(Q)@N-term) quite often in my bacterial proteomic experiments. I use a urea based buffer for the digestion. I was wondering if this purely biological modification or it occurs due to sample handling. If yes how can it be avoided ?
- Angiotensin Member
- Posts: 26
- Joined: Mon Mar 10, 2014 11:27 pm
The Gln->pyro-Glu(Q)@N-term reaction does happen spontaneously in vitro, at basic pH, even though at a slower rate than the enzyme-catalyzed reaction. I do not think you can avoid that.
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