Peptide modification: Gln->pyro-Glu(Q)@N-term

Share protocols and ask for sample preparation advice.
karthikskamath
Angiotensin Member
Angiotensin Member
Posts: 49
Joined: Thu Dec 15, 2011 7:58 am

Peptide modification: Gln->pyro-Glu(Q)@N-term

Postby karthikskamath » Wed Aug 17, 2016 4:44 pm

I have come across this modification (Gln->pyro-Glu(Q)@N-term) quite often in my bacterial proteomic experiments. I use a urea based buffer for the digestion. I was wondering if this purely biological modification or it occurs due to sample handling. If yes how can it be avoided ?

User avatar
zougman
Phosphoserine Member
Phosphoserine Member
Posts: 24
Joined: Mon Mar 10, 2014 11:27 pm

Re: Peptide modification: Gln->pyro-Glu(Q)@N-term

Postby zougman » Sat Aug 20, 2016 4:38 am

The Gln->pyro-Glu(Q)@N-term reaction does happen spontaneously in vitro, at basic pH, even though at a slower rate than the enzyme-catalyzed reaction. I do not think you can avoid that.


Return to “Sample Prep”

Who is online

Users browsing this forum: No registered users and 1 guest