Microcystin-LR

Share protocols and ask for sample preparation advice.
Arevik
Proton Member
Proton Member
Posts: 1
Joined: Thu Nov 29, 2012 6:17 am

Microcystin-LR

Postby Arevik » Thu Nov 29, 2012 11:54 pm

I need to extract microcystin-LR from cells in medium. what is the best way to do it? Acetic acid of 5 % for 1 hour? Dry-freezing and after bead biting to cause cells breakage? If I use cold and heat shock? Or to add some lysis buffer (like from DNA extraction kit with SDS)? and afterwards to go for filtration protocol?

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