Problems with Percolator in PD 1.3

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JHunter
Glycine Member
Glycine Member
Posts: 5
Joined: Fri Feb 22, 2013 2:01 pm

Problems with Percolator in PD 1.3

Postby JHunter » Wed Apr 03, 2013 5:54 am

Has anyone had problems with Percolator in PD1.3 using chymotrypsin?? I have successfully searched data with trypsin digests and Percolator worked fine, but when I search chymotrypsin data (all other parameters remain the same), Percolator fails. I tried decreasing the number of missed cleavages from 3 to 2 (we use 2 for our trypsin searches) but still got the same error...has anyone had this problem????

Torsten
Proton Member
Proton Member
Posts: 1
Joined: Thu Apr 04, 2013 12:53 pm

Postby Torsten » Thu Apr 04, 2013 1:00 pm

PD does not use any enzyme specific features at all, so from that it should make a difference which enzyme is used.
Without further details we cannot investigate this issue. We would need a bug report from the PD Bug Reporter tool sent to pd.support@thermofisher.com. You find the bug reporter under Tools > Create Bug Report... in PD.

-Torsten


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