one question about the fractionation and the protein identification number

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Sniper
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one question about the fractionation and the protein identification number

Postby Sniper » Wed Oct 30, 2013 6:17 pm

recently, i did an experiment that using an 50cm Colum coupled with tripToF5600, it takes 4 hours per experiment, the sample is ESCs, i want to know what is number of the factionation number is property for identification the most proteins in the sample, could anybody can give me some advise, or how can i using data analysis stratey to determin it? thanks a lot!

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