Identification of multiple proteins in a MALDI trypsin digest?

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AndorJKiss
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Identification of multiple proteins in a MALDI trypsin digest?

Postby AndorJKiss » Sat Oct 24, 2015 5:01 am

Hello,

This is a very nOOb question, but how does one go about identifying tryptic digest peptides from a mix of proteins. The mix is not complicated, perhaps two or three proteins. Would I use a program like Protein Prospector?

Any advice would be appreciated.

Thanks,
Andor

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zougman
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Postby zougman » Sat Oct 24, 2015 11:17 am

Hi Andor,
You can use MASCOT PMF search, for example. http://www.matrixscience.com/cgi/search_form.pl?FORMVER=2&SEARCH=PMF

AZ

AndorJKiss
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Postby AndorJKiss » Sun Oct 25, 2015 5:38 am

Hi,

I've tried that - what I'm looking for is a program that can separately assign the spectra to multiple proteins.

Regards,
Andor

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zougman
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Postby zougman » Sun Oct 25, 2015 7:05 am



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