I am preparing extraction buffer: 50mM Tris-HCl, pH 8.5, 5mM EDTA, 100mM KCl, 1% w/v dithiothreitol, 20% sucrose and protease inhibitor cocktail. I am just wondering, could you please check if I am doing it right way. I am preparing 100 ml extraction buffer
1) I prepared 50mM Tris-HCl , pH 8.5 (100 ml)
2)and then I added all these chemicals to the above 100 ml extraction buffer: 5mM EDTA (0.146g), 100mM KCl (0.745g), 1% w/v dithiothreitol (1g), 20% sucrose (20g) and appropriate amount of inhibitor cocktail.
Is this the right way of doing extraction buffer? Please check
1D, 2D, HPLC, SDS PAGE, etc. Talk about it here.
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