Single cell proteomics?

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Kambiz
Angiotensin Member
Angiotensin Member
Posts: 43
Joined: Tue Nov 29, 2011 12:34 am

Single cell proteomics?

Postby Kambiz » Sun Jun 08, 2014 8:22 pm

Hi everybody

I was wondering can we do single cell proteomics?

Motashkeram
Kambiz
Kambiz Gilany, Ph.D.
Assistant Professor
Avicenna Research Institute (ARI)

Evin, Tehran, Iran
P.O. Box: 19615-1177

Tel: +98-21-22432020 Ext. 427
Fax: +98 21 22432021

Website: http://www.avicenna.ac.ir/index.php/en

Kelstrup
Angiotensin Member
Angiotensin Member
Posts: 35
Joined: Fri Oct 05, 2012 5:19 am

Postby Kelstrup » Mon Jun 09, 2014 2:16 am


Kambiz
Angiotensin Member
Angiotensin Member
Posts: 43
Joined: Tue Nov 29, 2011 12:34 am

Sperm

Postby Kambiz » Sat Jun 14, 2014 2:26 am

You can isolate a single cell, specific human sperm. SO it is our instrumental that is not sensitive enough.
I am correct.

Motashekaram
Kambiz
Kambiz Gilany, Ph.D.
Assistant Professor
Avicenna Research Institute (ARI)

Evin, Tehran, Iran
P.O. Box: 19615-1177

Tel: +98-21-22432020 Ext. 427
Fax: +98 21 22432021

Website: http://www.avicenna.ac.ir/index.php/en

proteo14
Proton Member
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Posts: 2
Joined: Sat Nov 08, 2014 3:38 pm

Postby proteo14 » Sun Nov 23, 2014 6:51 am

Read this:

The single-probe: a miniaturized multifunctional device for single cell mass spectrometry analysis.
Pan N, Rao W, Kothapalli NR, Liu R, Burgett AW, Yang Z.
Anal Chem. 2014 Oct 7;86(19):9376-80. doi: 10.1021/ac5029038. Epub 2014 Sep 19.

Kelstrup
Angiotensin Member
Angiotensin Member
Posts: 35
Joined: Fri Oct 05, 2012 5:19 am

Postby Kelstrup » Mon Nov 24, 2014 3:53 am

proteo14 wrote:Read this:

The single-probe: a miniaturized multifunctional device for single cell mass spectrometry analysis.
Pan N, Rao W, Kothapalli NR, Liu R, Burgett AW, Yang Z.
Anal Chem. 2014 Oct 7;86(19):9376-80. doi: 10.1021/ac5029038. Epub 2014 Sep 19.


Thanks, think it shows some of the limitations quite well. I'm surprised no MS/MS data was shown, do you know why this was not done?


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