Mike Washburn interview

[Doug]

SharedProteomics talks with MudPUT developer and spectral counting expert Mike Washburn about the early days of MudPIT, the importance of biological replicates, and the use of spectral counting.

Read the interview here.

Leave comments and discuss the interview here.

[AAlpert]

Clicking on the link returns a message that I do not have access to this page.

[AAlpert]

OK, it's working now.

Not one word about the chromatography involved? All he talks about is that annoying detector downstream from the capillary...

[Doug]

Haha. Oh dear, chromatography is overlooked again. Well you know what they say, "Behind every great mass spectrometer..."

[ger225]

The question about dynamic exclusion causing spectral counting not work is spot on. I mean, I understand why people is often confused about DE and spectral counting. One can expect that after a peptide is fragmented causing an MS/MS event (a spectral count), then it shoud not appear once more. Well, it can be expected that it shows up every now and then, but I've seen datasets where the SpC for a peptide is in the hundreds! How can this be? How can a peptide trigger so many MS/MS events with DE on?