Peptide modification: Gln->pyro-Glu(Q)@N-term

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Peptide modification: Gln->pyro-Glu(Q)@N-term

Postby karthikskamath » Wed Aug 17, 2016 4:44 pm

I have come across this modification (Gln->pyro-Glu(Q)@N-term) quite often in my bacterial proteomic experiments. I use a urea based buffer for the digestion. I was wondering if this purely biological modification or it occurs due to sample handling. If yes how can it be avoided ?

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Re: Peptide modification: Gln->pyro-Glu(Q)@N-term

Postby zougman » Sat Aug 20, 2016 4:38 am

The Gln->pyro-Glu(Q)@N-term reaction does happen spontaneously in vitro, at basic pH, even though at a slower rate than the enzyme-catalyzed reaction. I do not think you can avoid that.

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