From a colleague:
We have a problem with our Easy nLC II from Thermo. The flow of pump B never stabilizes and it has fluctuations of +/- 200 nL/min. I have run the Purge Solvent and Flow Air functions of the maintenance section several times but it does not solve the problem. I ran the test that check for leakage and it passed. I also changed the rotor of the valve B and also the piston seal in pump B, but the problem persists. Now the flow of pump A started to have the same problem.
Has anyone seen something like this before? or have any suggestions as to what might be the cause?
1D, 2D, HPLC, SDS PAGE, etc. Talk about it here.
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- Angiotensin Member
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- Joined: Wed Jun 29, 2011 9:26 am
Based on the symptoms you describe, I would have thought that the rotor or piston seal was the problem. I don't remember what the problems were on our system, but at one point we had to replace the mixing tee and a few of the lines that feed in/out of that. I suppose that your flow sensor on B could have gone bad as well, but that seems less likely considering the new issues on A.
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