Coomassie Blue staining

Solutions

Staining solution

  • 50 mg Coomassie Blue R-250
  • 25 mL methanol
  • 20 mL water
  • 5 mL acetic acid

Destaining solution

  • 125 mL methanol
  • 100 mL water
  • 25 mL acetic acid

Procedure

  • Shake gel in staining solution for at least one hour
  • Destain by shaking with destaining solution (50 mL) 3–5× 30 min
    • Hint: adding KimWipes to the destaining solution can help speed this process
  • Wash gel with water 2–3× 5 min

Adapted from the Salk Institute Mass Spectrometry Core Facility (with permission)
Original document at http://cores.salk.edu/massspec/protocols/coomassie.php